Caspase-9 Antibody | 2073

Caspase-9 Antibody | 2073 | Gentaur UK, US & Europe Distribution

Host: Rabbit

Reactivity: Human, Mouse

Homology: Predicted species reactivity based on immunogen sequence: Rat (50%) .

Immunogen: Anti-Caspase 9 antibody (2073) was raised against a peptide corresponding to 20 amino acids near the center of human Caspase 9.
The immunogen is located within amino acids 290-340 of Caspase 9.

Research Area: Apoptosis, Cancer

Tested Application: E, WB, ICC, IF, IP

Application: WB: 1 μg/mL; ICC: 2- 5 μg/mL; IF: 5-20 μg/mL.
Antibody validated: Western Blot in human and mouse samples, Immunocytochemistry, Immunofluorescence in human samples. All other applications and species not yet tested.

Specificiy: Caspase-9 antibody is predicted to have no cross reactivity to other members in the caspase family.

Positive Control 1: Cat. No. 1201 - HeLa Cell Lysate

Positive Control 2: Cat. No. 17-004 - K-562 Cell Slide

Positive Control 3: N/A

Positive Control 4: N/A

Positive Control 5: N/A

Positive Control 6: N/A

Molecular Weight: Predicted: 46kD
Observed: 46 kD

Validation:

Independent Antibody Validation in Cell lines (Figure 1) shows similar CASPASE 9 expression profile in human cell lines detected by two independent anti-CASPASE 9 antibodies that recognize different epitopes, 2071 against N-terminus domain and 2073 against central domain.  CASPASE 9 proteins are detected in the most tested cell lines at different expression levels by the two independent antibodies.

Isoform: Human CASPASE 9 has 4 isoforms, including isoform 1 (416aa, 46kD) , isoform 2 (266aa, 30kD) , isoform 3 (154aa, 17kD) , and isoform 4 (333aa, 37kD) . Mouse CASPASE 9 has one isoform (454aa, 50kD) and Rat CASPASE 9 also has one isoform (454aa, 50kD) . 2073 can detect human and mouse isoform.

Purification: Caspase-9 Antibody is affinity chromatography purified via peptide column.

Clonality: Polyclonal

Clone: N/A

Isotype: IgG

Conjugate: Unconjugated

Physical State: Liquid

Buffer: Caspase-9 Antibody is supplied in PBS containing 0.02% sodium azide.

Concentration: 1 mg/mL

Storage Condition: Caspase-9 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Alternate Name: Caspase-9 Antibody: MCH6, APAF3, APAF-3, PPP1R56, ICE-LAP6, MCH6, Caspase-9, Apoptotic protease Mch-6, CASP-9

User Note: Optimal dilutions for each application to be determined by the researcher.

BACKGROUND: Caspase-9 Antibody: Apoptosis is related to many diseases and induced by a family of cell death receptors and their ligands. Cell death signals are transduced by death domain containing adapter molecules and members of the caspase family of proteases. A novel member in the caspase family was recently identified and designated ICE-LAP6, Mch6, and Apaf-3. Caspase-9 and Apaf-1 bind to each other, which leads to caspase-9 activation. Caspase-9 is also activated by granzyme B and CPP32. Activated caspase-9 cleaves and activates caspase-3 that is one of the key proteases, being responsible for the proteolytic cleavage of many key proteins in apoptosis. Caspase-9 play a central role in cell death induced by a wide variety of apoptosis activators including TNFα, TRAIL, anti-CD-95, FADD, and TRADD. Caspase-9 is expressed in a variety of human tissues.