Catalase, Human Recombinant | P1159

CAT is a key antioxidant enzyme in the bodies’ defense against oxidative stress. Also, it is a heme enzyme that is present in the peroxisome of nearly all aerobic cells. CAT converts the reactive oxygen species hydrogen peroxide to water and oxygen and thereby mitigates the toxic effects of hydrogen peroxide. Recombinant human CAT protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.

P1159 | Catalase, Human Recombinant DataSheet

Biomolecule/Target :CAT

Synonyms: CAT

Alternates names: CAT

Taglines: A key antioxidant enzyme in the bodies’ defense against oxidative stress

NCBI Gene ID #: 847

NCBI Gene Symbol: CAT

Gene Source: Human

Accession #: P04040

Recombinant: Yes

Source: E. coli

Purity by SDS-PAGE: ≥90%

Assay: SDS-PAGE

Purity: N/A

Assay #2: N/A

Endotoxin Level: N/A

Activity (Specifications/test method): Measured by its ability to decomposes 1.0 umole of H2O2 per minute at pH 7.0 at 25°C.

Biological activity: Specific activity is >20,000 unit/mg

Results: >20,000 unit/mg

Binding Capacity: N/A

Unit Definition: N/A

Molecular Weight: 62.5 kDa (547 aa, 1-552 aa + His Tag).

Concentration: N/A

Appearance: Lyophilized

Physical form description: Propriety buffer

Reconstitution Instructions: Centrifuge the vial prior to opening. Reconstitute in deionized water, store at 4°C and use within 2 weeks after reconstitution.

Amino acid sequence: MGSSHHHHHHSSGLVPRGSHMDDDDKADSRDPASDQMQHWKEQRAAQKADVLTTGAGNPVGDKLNVITVGPRGPLLVQDVVFTDEMAHFDRERIPERVVHAKGAGAFGYFEVTHDITKYSKAKVFEHIGKKTPIAVRFSTVAGESGSADTVRDPRGFAVKFYTEDGNWDLVGNNTPIFFIRDPILFPSFIHSQKRNPQTHLKDPDMVWDFWSLRPESLHQVSFLFSDRGIPDGHRHMNGYGSHTFKLVNANGEAVYCKFHYKTDQGIKNLSVEDAARLSQEDPDYGIRDLFNAIATGKYPSWTFYIQVMTFNQAETFPFNPFDLTKVWPHKDYPLIPVGKLVLNRNPVNYFAEVEQIAFDPSNMPPGIEASPDKMLQGRLFAYPDTHRHRLGPNYLHIPVNCPYRARVANYQRDGPMCMQDNQGGAPNYYPNSFGAPEQQPSALEHSIQYSGEVRRFNTANDDNVTQVRAFYVNVLNEEQRKRLCENIAGHLKDAQIFIQKKAVKNFTEVHPDYGSHIQALLDKYNAEKPKNAIHTFVQSGSHLAAREKANL

Handling: Centrifuge the vial prior to opening.

Usage: For Research Use Only! Not to be used in humans