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Cathepsin K, Active, rat recombinant | 7598

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SKU:
26-7598-GEN
Availability:
Usually shipped in 5 working days
£1,334.00 - £6,836.00

Description

Cathepsin K, a member of the papain cysteine proteinase family is the predominant proteinase responsible for the resorption of the bone matrix. Cathepsin cleaves proteins such as collagen type I, collagen type II and osteonectin, thereby playing a role in bone remodeling and resorption in osteoporosis, osteolytic bone metastasis and rheumatoid arthritis (Bromme and Okamoto, 1995; Drake, F. et al 1996; Bossard et al, 1996).

7598 | Cathepsin K Active rat recombinant DataSheet

Biomolecule/Target: Cathepsin K

Synonyms: CTSK, CTSO, CTSO3

Alternates names: MIC-1, Placental TGF, Prostate differentiation factor

Taglines: Predominant proteinase responsible for the resorption of the bone matrix.

NCBI Gene ID #: 9518

NCBI Gene Symbol: GDF-15

Gene Source: Rat

Accession #: Q99988

Recombinant: Yes

Source: E. coli

Purity by SDS-PAGEs: 95%

Assay: SDS-PAGE

Purity: N/A

Assay #2: N/A

Endotoxin Level: N/A

Activity (Specifications/test method): The Cathepsin K is fully active as seen from its ability to cleave Cathepsin K-specific fluorescent substrate using the Cathepsin K Activity assay kit (Cat # K141-100).

Biological activity: The activity is determined by the inhibition of DU-145 cells and is typically 1-2 µg/ml.

Results: The specific activity is >500 mU/mg (1U = 1 µmole/min/mg) as measured by Cathepsin K Activity Assay Kit (Catalog #K141-100)

Binding Capacity: N/A

Unit Definition: N/A

Molecular Weight: 26.8 kDa

Concentration: 0.5 mg/ml

Appearance: Liquid

Physical form description: 0.5 mg/ml solution in 25 mM Na2HPO4 and 500 mM NaCl (pH 7.0) with 50% glycerol.

Reconstitution Instructions: Centrifuge vial before opening. When reconstituting the product, gently pipet and wash down the sides of the vial to ensure full recovery of the protein into solution. It is recommended to reconstitute the lyophilized product with sterile 5 mM acetic acid at a concentration of 0.1 mg/ml, which can be further diluted into other aqueous solutions.

Amino acid sequence: MARNGDDCPL GPGRCCRLHT VRASLEDLGW ADWVLSPREV QVTMCIGACP SQFRAANMHA QIKTSLHRLK PDTVPAPCCV PASYNPMVLI QKTDTGVSLQ TYDDLLAKDC HCI

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Additional Information

Storage:
-80°C
Shipping:
Dry Ice
Shelf Life:
6 months
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