Cathepsin L Antibody | 3192

Cathepsin L is a normal lysosomal cysteine endoproteases that is expressed in all cells. The latent 39 kDa pro-Cathepsin L must be processed by cleavage of the 96 amino acid pro-peptide, to generate the active two-chain form made up of 21 and 5 kDa subunits. Mature Cathepsin L is normally localized in the lysosomes where it plays a major role in intracellular protein catabolism. The fact that Cathepsin L is also capable of degrading extracellular matrix proteins s?ggests that it may play a role in invasion and metastasis and could be a useful prognostic indicator for cancer.

3192 | Cathepsin L Antibody DataSheet

Antibody Target: Cathepsin L

Target Alternative Name: CTSL, CATL, CTSL1, MEP, EC 3.4.22.15

Tag Line:

Category: Cathepsin

Host: Rabbit

Isotype: Rabbit IgG

Species Reactivities: Human

Immunogen Sequence: Synthetic peptide surrounding amino acid 223 of human Cathepsin L

Accession #: P07711

Gene ID: 1514

Appearance: Colorless liquid

Form: Liquid

Concentration: 0.2 mg/ml

Formulation: 100 µg (0.2 mg/ml) affinity purified rabbit anti-cathepsin L polyclonal antibody in phosphate buffered saline (PBS), pH 7.2, containing 30% glycerol, 0.1% BSA, 0.01% thimerosal.

Purification: Affinity purified

Application: Western blot, Immunohistochemistry

Positive Control: Western Blot: Jurkat cell lysate. IHC: Kidney tissue

Application And Usages: Western blotting (0.5-4 µg/ml), and Immunohistochemistry (5 µg/ml). However, the optimal conditions should be determined individually. Jurkat cell lysate (Cat.# 2401-100) can be used as a positive control. Detects both the proenzyme and the mature form of cathepsin L

Country of Animal Origin: China

Country of Manufacture: USA

Usage: For Research Use Only! Not to be used in humans.

Handling: The antibody solution should be gently mixed before use.

Western Blot Verified: Verified

Immunocytochemistry Verified: Verified

Immunofluorescence Verified: Not Verified

Immunoprecipitation Verified: Not Verified

FACS Verified:

ELISA Verified: Not Verified

ChIP Verified: Not Verified

Dot Blot Verified:

Flow Cytometry Verified: Not Verified