Cathepsin L, human recombinant | 1135

Cathepsin L is a lysosomal protease that can be secreted from the cell and is implicated to play a role in protein degradation, arthritis, apoptosis, and cancer.

1135 | Cathepsin L human recombinant DataSheet

Biomolecule/Target: Cathepsin L

Synonyms: Cathepsin

Alternates names: MIC-1, Placental TGF, Prostate differentiation factor

Taglines: Lysosomal cysteine protease involved in protein degradation, arthritis, apoptosis, and cancer

NCBI Gene ID #: 9518

NCBI Gene Symbol: GDF-15

Gene Source: Human

Accession #: Q99988

Recombinant: Yes

Source: E. coli

Purity by SDS-PAGEs: 95%

Assay: SDS-PAGE

Purity: N/A

Assay #2: HPLC

Endotoxin Level: N/A

Activity (Specifications/test method): > 10000 mU/mg (1 U = digestion of 1 µmole of Z-Phe-Arg-AFC substrate (K142-100) per minute at 37°C in 50 mM Sodium Acetate, 100 mM NaCl, pH 5.5).

Biological activity: The activity is determined by the inhibition of DU-145 cells and is typically 1-2 µg/ml.

Results: > 10000 mU/mg

Binding Capacity: N/A

Unit Definition: 1 U = digestion of 1 µmole of Z-Phe-Arg-AFC substrate (K142-100) per minute at 37°C in 50 mM Sodium Acetate, 100 mM NaCl, pH 5.5

Molecular Weight: 26.8 kDa

Concentration: 0.5 mg/ml

Appearance: Liquid

Physical form description: 0.5 mg/ml in 25 mM Sodium Acetate, 50 mM NaCl, pH 5.5 containing 50% glycerol

Reconstitution Instructions: Centrifuge vial before opening. When reconstituting the product, gently pipet and wash down the sides of the vial to ensure full recovery of the protein into solution. It is recommended to reconstitute the lyophilized product with sterile 5 mM acetic acid at a concentration of 0.1 mg/ml, which can be further diluted into other aqueous solutions.

Amino acid sequence: MARNGDDCPL GPGRCCRLHT VRASLEDLGW ADWVLSPREV QVTMCIGACP SQFRAANMHA QIKTSLHRLK PDTVPAPCCV PASYNPMVLI QKTDTGVSLQ TYDDLLAKDC HCI