Cattle AMH (Anti-Mullerian Hormone) ELISA Kit | ELK7255

Cattle AMH (Anti-Mullerian Hormone) ELISA Kit | ELK7255 | Gentaur UK, US & Europe Distribution

Alternative Names: MIF; MIH; MIS; Müllerian Inhibiting Factor; Müllerian Inhibiting Hormone; Müllerian Inhibiting Substance

Species: Cattle

Assay Type: Competitive Inhibition

Sensitivity: 177.4 pg/mL

Standard: 40000 pg/mL

Detection Range: 625-40000 pg/mL

Sample Type: Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids

Assay Lenght: 2h

Research Area: Endocrinology;Reproductive science;Hormone metabolism

Test Principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Anti-Mullerian Hormone (AMH) protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Anti-Mullerian Hormone (AMH) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Anti-Mullerian Hormone (AMH) in the samples is then determined by comparing the OD of the samples to the standard curve.