Cattle AP (Aprotinin) ELISA Kit | ELK6113

Cattle AP (Aprotinin) ELISA Kit | ELK6113 | Gentaur UK, US & Europe Distribution

Alternative Names: BPTI; Trasylol; Pancreatic Trypsin Inhibitor; Basic protease inhibitor

Species: Cattle

Assay Type: Competitive Inhibition

Sensitivity: 0.55 ng/mL

Standard: 100 ng/mL

Detection Range: 1.57-100 ng/mL

Sample Type: serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids

Assay Lenght: 2h

Research Area: Enzyme & Kinase;Cardiovascular biology;Human gene deletion;Hepatology

Test Principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Aprotinin (AP) protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Aprotinin (AP) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Aprotinin (AP) in the samples is then determined by comparing the OD of the samples to the standard curve.