Cattle HB (Hemoglobin) ELISA Kit | ELK8165

Cattle HB (Hemoglobin) ELISA Kit | ELK8165 | Gentaur UK, US & Europe Distribution

Alternative Names: Hgb; Haemoglobin; Heterotetramer (αβ) 2

Species: Cattle

Assay Type: Competitive Inhibition

Sensitivity: 5.57 μg/mL

Standard: 1000 μg/mL

Detection Range: 15.63-1000 μg/mL

Sample Type: Serum, plasma, erythrocyte lysates and other biological fluids

Assay Lenght: 2h

Research Area: Metabolic pathway;Infection immunity;Hematology;Neuro science

Test Principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Hemoglobin (HB) protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Hemoglobin (HB) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Hemoglobin (HB) in the samples is then determined by comparing the OD of the samples to the standard curve.