Cattle PF4 (Platelet Factor 4) ELISA Kit | ELK5909

Cattle PF4 (Platelet Factor 4) ELISA Kit | ELK5909 | Gentaur UK, US & Europe Distribution

Alternative Names: CXCL4; SCYB4; Chemokine C-X-C-Motif Ligand 4; Oncostatin-A; Iroplact

Species: Cattle

Assay Type: Sandwich

Sensitivity: 2.95 pg/mL

Standard: 400 pg/mL

Detection Range: 6.25-400 pg/mL

Sample Type: Serum, platelet-poor plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids

Assay Lenght: 3.5h

Research Area: Cytokine;Hematology

Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Platelet Factor 4 (PF4) . Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Platelet Factor 4 (PF4) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Platelet Factor 4 (PF4), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Platelet Factor 4 (PF4) in the samples is then determined by comparing the OD of the samples to the standard curve.