Cattle SPATA4 (Spermatogenesis-associated protein 4) ELISA Kit | ELK8999

Cattle SPATA4 (Spermatogenesis-associated protein 4) ELISA Kit | ELK8999 | Gentaur UK, US & Europe Distribution

Alternative Names: SPATA4

Species: Cattle

Assay Type: Sandwich

Sensitivity: 0.061 ng/mL

Standard: 10 ng/mL

Detection Range: 0.16-10 ng/mL

Sample Type: serum, plasma, tissue homogenates

Assay Lenght: 3.5h

Research Area: Reproductive science

Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Spermatogenesis-associated protein 4 (SPATA4 ) . Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Spermatogenesis-associated protein 4 (SPATA4 ) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Spermatogenesis-associated protein 4 (SPATA4 ), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Spermatogenesis-associated protein 4 (SPATA4 ) in the samples is then determined by comparing the OD of the samples to the standard curve.