CCL25 (Human) ELISA Kit | E5120-100

CCL25 (Human) ELISA Kit | E5120-100

Description: CCL25 is a small cytokine belonging to the CC chemokine family that is also known as TECK (Thymus-Expressed Chemokine). It shows chemotactic activity on thymocytes, macrophages, THP-1 cells, and dendritics cells but is inactive on peripheral blood lymphocytes and neutrophils. CCL25 elicits its effects by binding to the chemokine receptor CCR9. It binds to atypical chemokine receptor ACKR4 and mediates the recruitment of beta-arrestin (ARRB1/2) to ACKR4. CCL25 acts via the Gαi-coupled receptor CCR9 to impair beta-cell function by inhibiting insulin secretion and promoting cytokine-induced apoptosis. Following treatment with CCL25, lung cancer cells demonstrated higher migratory and invasive potential, which could be blocked by the CCR9 antibody. CCR9/CCL25 has been shown to play an important role in a variety of inflammation-related diseases, such as cardiovascular disease (CVD), rheumatoid arthritis, hepatitis, inflammatory bowel diease, asthma, etc. ’s CCL25 (Human) ELISA Kit is used for the quantitative detection of CCL25 in serum, plasma, tissue homogenates and other biological fluids. It is based on the principle of sandwich ELISA. The capture antibody is pre-coated on 96-well plates. The standards, test samples and biotin conjugated detection antibody are added to the wells subsequently, and washed with wash buffer. HRP-Streptavidin is added and unbound conjugates are washed away with wash buffer. The HRP enzymatic reaction is detected using TMB-substrate. Finally, an acidic stop solution terminates the enzymatic reaction. The color developed is directly proportional to the amount of CCL25 in the sample.

Alternate Name: CCL25 ELISA Kit; TECK ELISA Kit; Thymus Expressed Chemokine ELISA Kit

Tag Line: A highly sensitive sandwich ELISA kit for the quantitative measurement of CCL25

Summary: N/A

Detection Method: Absorbance is measured at 450 nm

Sample Type: HumaSerum, Plasma, Tissue lysates and other biological fluids

Species Reactivity: Human

Applications: The ELISA kit is used for the quantitative detection of CCL25 in serum, plasma, tissue homogenates and other biological fluids

Features & Benefits: Highly sensitive and specific Detection range: 62.5-4000 pg/ml Assay Precision; Intra-Assay CV < 8% and Inter-Assay < 10% Recovery Rate: 85-101% for serum, 87-93% for EDTA plasma and 94-100% for heparin plasma Sensitivity: 37.5 pg/ml