CD-14, human recombinant | 4937

CD14 acts as a receptor for endotoxin (LPS), is expressed strongly on monocy¬tes, macro¬pha¬ges and weekly at surface of neutrophiles. CD14 is anchored to cells by linkage to glycosylphosphatidylinositol (GPI) and functions as a high affinity receptor LPS-LBP (lipopolysaccharide binding protein)-complexes. Furthermore CD14 is present in a soluble form in human serum, urine and other body fluids, acts at physiological concentrations as LPS agonist and has at higher concentrations an antagonizing effect in cell activation. The myeloid differentiation antigen CD14 acts as the major receptor for bacterial LPS. The dominant form of the recombinant wild type CD14 is the 50 kDa protein.

4937 | CD-14 human recombinant DataSheet

Biomolecule/Target: N/A

Synonyms: CD14, Cluster of differentiation 14, Human CD14, CD14, h-CD14, rh-CD14, recombinant human CD14, recombinant CD14, CD14

Alternates names: CD14, Cluster of differentiation 14, Human CD14, CD14, h-CD14, rh-CD14, recombinant human CD14, recombinant CD14, CD14, Monocyte differentiation antigen CD14, Myeloid cell-specific leucine-rich glycoprotein

Taglines: A high affinity receptor for complexes of LPS and LPS binding protein

NCBI Gene ID #: 627

NCBI Gene Symbol: BDNF

Gene Source: Human

Accession #: P23560

Recombinant: Yes

Source: CHO cells

Purity by SDS-PAGEs: 98%

Assay: SDS-PAGE

Purity: N/A

Assay #2: HPLC

Endotoxin Level: <0.1 ng/g

Activity (Specifications/test method): N/A

Biological activity: Inhibits binding of LPS to CD14. Upto to 20 µg/ml CD14 inhibit binding of FITC-LPS (0.5µg/ml) to 6 x 105 CD14+CHO transfectants (FACS)

Results: N/A

Binding Capacity: N/A

Unit Definition: N/A

Molecular Weight: 50.0 kDa

Concentration: N/A

Appearance: Lyophilized protein

Physical form description: Lyophilized from a concentrated protein solution (1 mg/ml) containing PBS, pH 7.2

Reconstitution Instructions: Reconstitute in 10 µl of ultra-pure distilled water. This solution can then be diluted into other aqueous buffers and stored at –20°C or –70°C for future use.

Amino acid sequence: N/A