CD11b Antibody [M1/70] (Violet-450) | 76-122

CD11b Antibody [M1/70] (Violet-450) | 76-122 | Gentaur UK, US & Europe Distribution

Host: Rat

Reactivity: Human, Mouse

Homology: N/A

Immunogen: N/A

Research Area: Innate Immunity

Tested Application: Flow

Application: N/A

Specificiy: The M1/70 monoclonal antibody specifically reacts with the 170 kDa alphaM integrin chain of mouse CD11b from the Mac-1 integrin (CD11b/CD18) .

Positive Control 1: N/A

Positive Control 2: N/A

Positive Control 3: N/A

Positive Control 4: N/A

Positive Control 5: N/A

Positive Control 6: N/A

Molecular Weight: N/A

Validation: N/A

Isoform: N/A

Purification: The monoclonal antibody was purified utilizing affinity chromatography and unreacted dye was removed from the product.

Clonality: Monoclonal

Clone: M1/70

Isotype: Rat IgG2b, kappa

Conjugate: Violet-450

Physical State: liquid

Buffer: Phosphate-buffered aqueous solution, ≤0.09% Sodium azide, may contain carrier protein/stabilizer, ph7.2.

Concentration: batch dependent

Storage Condition: The product should be stored undiluted at 4˚C and should be protected from prolonged exposure to light. Do not freeze.

Alternate Name: CR3A, MO1A, CD11B, MAC-1, MAC1A, SLEB6, ITGAM

User Note: Optimal dilutions for each application to be determined by the researcher.

BACKGROUND: The M1/70 monoclonal antibody specifically reacts with the 170 kDa alphaM integrin chain of mouse CD11b from the Mac-1 integrin (CD11b/CD18) . Mac-1 binds to C3bi, CD54 (ICAM-1) , and fibrinogen, and it is expressed by granulocytes, macrophages, NK cells, myeloid-derived dendritic cells, microglia, activated lymphocytes, and mouse B-1 cells. The expression is up-regulated on activated neutrophils at the same time that L-selectin is shed from the cell surface. The M1/70 antibody is used for the detection of monocytes, granulocytes, and a subset of natural killer cells in human peripheral blood.M1/70 blocks C3bi binding and cell adherence, but not cell-mediated lysis and it cross-reacts with human CD11b.BG Violet 450 conjugate is an alternative to the Pacific Blue, eFluor 450, or BD Horizon V450 dyes. It is excited by the violet (405 nm) laser, with a peak emission of 450nm.