CD16a/Fc gamma RIIIA | E21-441

CD16a/Fc gamma RIIIA | E21-441 | Gentaur UK, US & Europe Distribution

Short Description: Recombinant Human Low Affinity Immunoglobulin Gamma Fc Region Receptor III-A is produced by our Mammalian expression system and the target gene encoding Gly17-Gln208 is expressed with a 6His tag at the C-terminus.

Shipping: Ambient

Formulation: Lyophilized from a 0.2 m filtered solution of 20mM PB, 150mM NaCl, pH 7.2.

Storage: Lyophilized protein should be stored at < -20C, though stable at room temperature for 3 weeks.
Reconstituted protein solution can be stored at 4-7C for 2-7 days.
Aliquots of reconstituted samples are stable at < -20C for 3 months.

Reconstitution: Always centrifuge tubes before opening. Do not mix by vortex or pipetting.
It is not recommended to reconstitute to a concentration less than 100 g /ml.
Dissolve the lyophilized protein in ddH2O.
Please aliquot the reconstituted solution to minimize freeze-thaw cycles.

Purity: Greater than 95% as determined by reducing SDS-PAGE.

Endotoxin: Less than 0.1 ng/g (1 IEU/g) as determined by LAL test.

Background: Receptors for the Fc region of immunoglobin G (FcR) are divided into three classes and FcRIII is a multifunctional, low/intermediate affinity receptor. In humans, FcRIII is expressed as two distinct forms (FcRIIIA and FcRIIIB) that are encoded by two different but highly homologous genes in a cell type-specific manner. FcRIIIB is a low-affinity, GPI-linked receptor expressed by neutrophils and eosinophils, whereas FcRIIIA is an intermediate affinity polypeptide-anchored transmembrane glycoprotein expressed by a subset of T lymphocytes, natural killer (NK) cells, monocytes, and macrophages. The FcRIIIA receptor is involved in phagocytosis, secretion of enzymes, inflammatory mediators, antibody-dependent cellular cytotoxicity (ADCC) , mast cell degranulation, and clearance of immune complexes. FcRIIIA has an immunoreceptor tyrosine-based activation motif (ITAM) in its cytoplasmic domain and delivers an activation signal in the immune responses. Aberrant expression or mutations in this gene is implicated in susceptibility to recurrent viral infections, systemic lupus erythematosus, and alloimmune neonatal neutropenia. In humans, it is a 50 -70 kD type I transmembrane activating receptor. The FcRIIIA cDNA encodes 254 amino acid including a 16aa signal sequence, 191 amino acid ECD with two C2-type Ig-like domains, five potential N-glycosylation sites, a 22 amino acid transmembrane sequence and a 25 amino acid cytoplasmic domain.

Species: Human

Expression System: Human cells