CD28 Antibody [AV7] (Biotin) | 99-238

CD28 Antibody [AV7] (Biotin) | 99-238 | Gentaur UK, US & Europe Distribution

Host: Mouse

Reactivity: Chicken, Turkey

Homology: N/A

Immunogen: Chicken peripheral blood lymphocytes

Research Area: Immunology, Signal Transduction

Tested Application: IHC-Fr, Flow, Blocking, IP, Complement Mediated Cell Depletion

Application: CD28 Antibody [AV7] for use in flow cytometry, immunohistochemistry / immunocytochemistry, and immunoprecipitation assays.

Specificiy: CD28

Positive Control 1: N/A

Positive Control 2: N/A

Positive Control 3: N/A

Positive Control 4: N/A

Positive Control 5: N/A

Positive Control 6: N/A

Molecular Weight: N/A

Validation: N/A

Isoform: N/A

Purification: N/A

Clonality: Monoclonal

Clone: [AV7]

Isotype: IgG1

Conjugate: Biotin

Physical State: Liquid

Buffer: Supplied in PBS/NaN3

Concentration: 0.5 mg/mL

Storage Condition: Store vial at 2-8˚C

Alternate Name: N/A

User Note: Optimal dilutions for each application to be determined by the researcher.

BACKGROUND: Chicken CD28 antigen is a type I transmembrane monomeric glycoprotein which does not contain the cysteine residues that form disulfide-linked covalent homodimers found in the mammalian counterpart. This may reduce the avidity of binding to individual ligand molecules expected to be present on antigen presenting cells. Peripheral gammadelta+ T cells are CD28 negative. Monoclonal antibodies against CD28 have a costimulatory effect on T cells stimulated by phorbol myristate acetate (PMA) , concanavalin A, or monoclonal antibodies against the avian T cell receptor (TCR) . Both Vbeta1- and Vbeta2-expressing cells respond equally well to stimulation with anti-CD28 in combination with PMA. These responses are resistant to cyclosporin A but inhibited by herbimycin A suggesting that CD28 employs a signaling pathway at least partly distinct from that triggered by TCR/CD3. The monoclonal antibody AV7 also reacts with turkey CD28 as demonstrated by flow cytometry.