CD3e Antibody [CRIS-7] | 33-484

CD3e Antibody [CRIS-7] | 33-484 | Gentaur UK, US & Europe Distribution

Host: Mouse

Reactivity: Human, Monkey

Homology: N/A

Immunogen: Stimulated human leukocytes were used as the immunogen for the CD3e antibody.

Research Area: Cancer, Immunology, Signal Transduction

Tested Application: Func, Flow, IF

Application: Functional Activity: Induce T cell activation and proliferation (Order SAF formulation)
Flow Cytometry: 0.5-1 ug/million cells in 0.1ml
Immunofluorescence: 1-2 ug/ml
Optimal dilution of the CD3e antibody should be determined by the researcher.

Specificiy: N/A

Positive Control 1: N/A

Positive Control 2: N/A

Positive Control 3: N/A

Positive Control 4: N/A

Positive Control 5: N/A

Positive Control 6: N/A

Molecular Weight: N/A

Validation: N/A

Isoform: N/A

Purification: Protein G affinity chromatography

Clonality: Monoclonal

Clone: CRIS-7

Isotype: IgG2a, kappa

Conjugate: Unconjugated

Physical State: Liquid

Buffer: PBS with 0.1 mg/ml BSA and 0.05% sodium azide

Concentration: 0.2 mg/mL

Storage Condition: Aliquot and Store at 2-8˚C. Avoid freez-thaw cycles.

Alternate Name: T-cell surface glycoprotein CD3 epsilon chain, T-cell surface antigen T3/Leu-4 epsilon chain, CD3e, CD3E, T3E

User Note: Optimal dilutions for each application to be determined by the researcher

BACKGROUND: Recognizes the epsilon-chain of CD3 (Workshop V; Code: CD03.09) , which consists of five different polypeptide chains (designated as gamma, delta, epsilon, zeta, and eta) with MW ranging from 16-28kDa. The CD3 complex is closely associated at the lymphocyte cell surface with the T cell antigen receptor (TCR) . Reportedly, CD3 complex is involved in signal transduction to the T cell interior following antigen recognition. The CD3 antigen is first detectable in early thymocytes and probably represents one of the earliest signs of commitment to the T cell lineage. In cortical thymocytes, CD3 is predominantly intra-cytoplasmic. However, in medullary thymocytes, it appears on the T cell surface. CD3 antigen is a highly specific marker for T cells, and is present in majority of T cell neoplasms.