CD3Z Antibody (N-term) [APR15350G]

CD3Z Antibody (N-term) [APR15350G] | Gentaur UK, US & Europe Distribution

Product Category: Polyclonal Antibodies

Host: Rabbit

Species Reactivity: H

Specificity: This CD3Z antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 2-31 amino acids from the N-terminal region of human CD3Z.

Cellular Localisation: Cell membrane {ECO:0000250|UniProtKB:P24161}; Single-pass type I membrane protein

Molecular Weight: 18696

Clone: Polyclonal

Gene Name: CD247

Gene ID: 919

Function: Part of the TCR-CD3 complex present on T-lymphocyte cell surface that plays an essential role in adaptive immune response. When antigen presenting cells (APCs) activate T-cell receptor (TCR), TCR- mediated signals are transmitted across the cell membrane by the CD3 chains CD3D, CD3E, CD3G and CD3Z. All CD3 chains contain immunoreceptor tyrosine-based activation motifs (ITAMs) in their cytoplasmic domain. Upon TCR engagement, these motifs become phosphorylated by Src family protein tyrosine kinases LCK and FYN, resulting in the activation of downstream signaling pathways (PubMed:2470098, PubMed:7509083) . CD3Z ITAMs phosphorylation creates multiple docking sites for the protein kinase ZAP70 leading to ZAP70 phosphorylation and its conversion into a catalytically active enzyme (PubMed:7509083) . Plays an important role in intrathymic T-cell differentiation. Additionally, participates in the activity-dependent synapse formation of retinal ganglion cells (RGCs) in both the retina and dorsal lateral geniculate nucleus (dLGN) (By similarity) .

Summary: Tissue Location: CD3Z is expressed in normal lymphoid tissue and in peripheral blood mononuclear cells (PBMCs) (PubMed:11722641)

Form: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.

Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

Application: WB, FC

Dilution: WB--1:1000 FC--1:10~50

Synonyms: T-cell surface glycoprotein CD3 zeta chain, T-cell receptor T3 zeta chain, CD247, CD247, CD3Z, T3Z, TCRZ