CD4 Antibody [9H5A8] | PM-5201

CD4 Antibody [9H5A8] | PM-5201 | Gentaur UK, US & Europe Distribution

Host: Mouse

Reactivity: Human, Mouse, Rat

Homology: N/A

Immunogen: Mouse monoclonal CD4 antibody was raised against a 193 amino acid recombinant protein from near the amino terminus of human CD4.

Research Area: Immunology

Tested Application: E, WB, IHC-P

Application: WB: 0.5-4 μg/mL; IHC: 5 μg/mL.
Antibody validated: Western Blot in human, mouse and rat samples; Immunohistochemistry in human samples. All other applications and species not yet tested.

Specificiy: N/A

Positive Control 1: Cat. No. 1314 - Human Thymus Tissue Lysate

Positive Control 2: Cat. No. 1205 - Jurkat Cell Lysate

Positive Control 3: N/A

Positive Control 4: N/A

Positive Control 5: N/A

Positive Control 6: N/A

Molecular Weight: Predicted: 51kD
Observed: 51-57 kD (Post-modyfication: 2-4 N-linked glycosylations)

Validation:

Recombinant Protein Test (Figure 1) : Anti-CD4 antibodies (PM-5201) detected human CD-4 recombinant protein at 5ng.

Isoform: Human CD4 has one isoform (458aa, 51kD) . Mouse CD4 has two isoforms, including isoform 1 (457aa, 51kD) and isoform 2 (217aa, 24.5kD) . Rat CD4 has one isoform (457aa, 51kD) . PM-5201 can detect human, mouse and rat.

Purification: CD4 Monoclonal Antibody is immunoaffinity chromotography purified IgG.

Clonality: Monoclonal

Clone: 9H5A8

Isotype: IgG1

Conjugate: Unconjugated

Physical State: Liquid

Buffer: CD4 Monoclonal Antibody is supplied in PBS containing 0.02% sodium azide.

Concentration: 1 mg/mL

Storage Condition: CD4 monoclonal antibody can be stored at -20˚C, stable for one year.

Alternate Name: CD4 Antibody [9H5A8] : CD4mut

User Note: Optimal dilutions for each application to be determined by the researcher.

BACKGROUND: CD4 Monoclonal Antibody: CD4 is a member of the immunoglobulin superfamily and is implicated as associative recognition element in MHC (Major Histocompatibility Complex) class II-restricted immune response. On T-lymphocytes, it defines the helper/inducer subset. The mature 55 kd CD4 protein consists of a 372 amino acid extracellular segment composed of four tandem immunoglobulin-like VJ regions. The CD4 molecule is a major receptor for human immunodeficiency virus (HIV) , binding directly to the envelope glycoprotein gp120 on HIV, with the co-receptors being CCR5 or CXCR4. It has been shown that the V-like domains are critical for binding with HIV envelope gp120.