CD45RO Antibody [190-2F2.5] | 33-301

CD45RO Antibody [190-2F2.5] | 33-301 | Gentaur UK, US & Europe Distribution

Host: Mouse

Reactivity: Human

Homology: N/A

Immunogen: Lentillectin purified glycoproteins obtained from HuPBL and from a CLL (B cell) were used as immunogen for this CD45RO antibody.

Research Area: Cancer, Immunology, Neuroscience, Signal Transduction

Tested Application: WB, Flow, IF

Application: Flow Cytometry: 0.5-1 ug/million cells
Immunofluorescence: 1-2 ug/ml
Due to variation in protocol and secondary antibody used, the CD45RO antibody may need to be titered for optimal performance.

Specificiy: N/A

Positive Control 1: N/A

Positive Control 2: N/A

Positive Control 3: N/A

Positive Control 4: N/A

Positive Control 5: N/A

Positive Control 6: N/A

Molecular Weight: N/A

Validation: N/A

Isoform: N/A

Purification: Protein G affinity chromatography

Clonality: Monoclonal

Clone: 190-2F2.5

Isotype: IgG2a, kappa

Conjugate: Unconjugated

Physical State: Liquid

Buffer: PBS with 0.1 mg/ml BSA and 0.05% sodium azide

Concentration: 0.2 mg/mL

Storage Condition: Aliquot and Store at 2-8˚C. Avoid freez-thaw cycles.

Alternate Name: Receptor-type tyrosine-protein phosphatase C, Leukocyte common antigen, L-CA, T200, CD45, PTPRC, CD45

User Note: Optimal dilutions for each application to be determined by the researcher

BACKGROUND: Recognizes a 180-185kDa protein, identified as isoform of leukocyte common antigen (CD45RO) . This antibody reacts with mature activated T-cells, most thymocytes, and a sub-population of resting T-cells within both CD4 and CD8 subsets. It shows no reactivity with normal B or natural killer cells, but reacts with granulocytes and monocytes. Reportedly, it is useful to identify T-cell lymphomas and leukemias. It rarely stains NK cells or B-cell lymphomas.