CD54 Antibody [15.2] (FITC) | 99-389

CD54 Antibody [15.2] (FITC) | 99-389 | Gentaur UK, US & Europe Distribution

Host: Mouse

Reactivity: Human, Pig

Homology: N/A

Immunogen: Rheumatoid synovial cells and human monocytes

Research Area: Immunology, Signal Transduction

Tested Application: Flow

Application: CD54 Antibody [15.2] for use in flow cytometry, immunohistochemistry / immunocytochemistry, and western blotting assays.

Specificiy: CD54

Positive Control 1: N/A

Positive Control 2: N/A

Positive Control 3: N/A

Positive Control 4: N/A

Positive Control 5: N/A

Positive Control 6: N/A

Molecular Weight: N/A

Validation: N/A

Isoform: N/A

Purification: N/A

Clonality: Monoclonal

Clone: [15.2]

Isotype: IgG1

Conjugate: FITC

Physical State: Liquid

Buffer: Supplied in PBS/NaN3

Concentration: Lot specific

Storage Condition: Store vial at 2-8˚C

Alternate Name: ICAM-1, intracellular adhesion molecule-1, Ly-47

User Note: Optimal dilutions for each application to be determined by the researcher.

BACKGROUND: CD54, also known as intercellular cell adhesion molecule-1 (ICAM-1) , is a 90 kDa type I transmembrane glycoprotein and a member of the immunoglobulin superfamily. It exhibits a wide tissue distribution being expressed on both hematopoietic and non-hematopoietic cells. ICAM-1 expression on leukocytes is low but is rapidly upregulated upon activation. Expression on endothelium and other non-hematopoietic cells is strongly upregulated by inflammatory mediators. The ligands of CD54 are CD11a/LFA-1alpha and CD11b/Mac-1alpha. Endothelial CD54 contributes to the extravasation of leukocytes from blood vessels particularly in areas of inflammation. CD54 on antigen-presenting cells (APC) contributes to antigen-specific T cell activation presumably by enhancing interactions between T cells and APC.