CD8a Antibody [53-6.7] (PE-Texas Red) | 98-617

CD8a Antibody [53-6.7] (PE-Texas Red) | 98-617 | Gentaur UK, US & Europe Distribution

Host: Rat

Reactivity: Mouse

Homology: N/A

Immunogen: Spleen cells or thymocyte membranes

Research Area: Immunology

Tested Application: E, WB, IHC-P, IHC-Fr, ICC, Flow, IP, Neut, Multiplex

Application: CD8a Antibody [53-6.7] for use in flow cytometry assays.

Specificiy: CD8alpha

Positive Control 1: N/A

Positive Control 2: N/A

Positive Control 3: N/A

Positive Control 4: N/A

Positive Control 5: N/A

Positive Control 6: N/A

Molecular Weight: N/A

Validation: N/A

Isoform: N/A

Purification: N/A

Clonality: Monoclonal

Clone: [53-6.7]

Isotype: IgG2a

Conjugate: PE-Texas Red

Physical State: Liquid

Buffer: Supplied in PBS/NaN3 and a stabilizing agent

Concentration: 0.1 mg/mL

Storage Condition: Store vial at 2-8˚C

Alternate Name: Ly-2, Lyt-2, Ly-35, Ly-B, T8

User Note: Optimal dilutions for each application to be determined by the researcher.

BACKGROUND: In the mouse, CD8 exists in two forms ��� (i) a CD8 heterodimer composed of an alpha chain (CD8alpha) and a beta chain (CD8beta) ; and (ii) a homodimer of two alpha chains. The heterodimer is found on the surface of essentially all thymocytes and the ���suppressor/cytotoxic��� subpopulation of mature T lymphocytes. Subsets of intestinal intraepithelial lymphocytes express CD8alpha without CD8beta. It has been suggested that CD8+beta- T cells mature extrathymically, while development of the CD8alpha+beta+ population of T cells is thymus-dependent. CD8 acts as a coreceptor with MHC Class I-restricted T cell receptors in antigen recognition and positive selection of MHC class I-restricted CD8+ T cells. In vivo and in vitro treatment with the 53-6.7 monoclonal antibody effectively depletes CD8alpha+ cells. The 53-6.7 monoclonal antibody also blocks allogeneic help specific for class I MHC antigens and T cell responses to IL-2.