CD8a Antibody [CT-8] (APC) | 99-216

CD8a Antibody [CT-8] (APC) | 99-216 | Gentaur UK, US & Europe Distribution

Host: Mouse

Reactivity: Chicken, Turkey, Guinea Fowl, Pigeon

Homology: N/A

Immunogen: Chicken thymocytes and Ig-negative blood leukocytes

Research Area: Cancer, Immunology, Signal Transduction

Tested Application: Flow

Application: CD8a Antibody [CT-8] for use in flow cytometry assays.

Specificiy: CD8alpha

Positive Control 1: N/A

Positive Control 2: N/A

Positive Control 3: N/A

Positive Control 4: N/A

Positive Control 5: N/A

Positive Control 6: N/A

Molecular Weight: N/A

Validation: N/A

Isoform: N/A

Purification: N/A

Clonality: Monoclonal

Clone: [CT-8]

Isotype: IgG1

Conjugate: APC

Physical State: Liquid

Buffer: Supplied in PBS/NaN3 and a stabilizing agent

Concentration: 0.1 mg/mL

Storage Condition: Store vial at 2-8˚C

Alternate Name: N/A

User Note: Optimal dilutions for each application to be determined by the researcher.

BACKGROUND: In the chicken, the CD8 molecule is present in two forms - (i) a homodimer of two alpha chains and (ii) a heterodimer of an alpha chain and a beta chain. While the vast majority of CD8+ cells in the thymus, spleen, and blood of adult chickens express both CD8alpha- and CD8beta-chains, a relatively large proportion of the CD8+ TCRgammadelta cells in the spleens of embryos and young chicks express only the alpha-chain of CD8. Among intestinal epithelial lymphocytes, the major CD8+ T cell populations present in mice are conserved but there is a population of TCRgammadelta CD8alphabeta cells in the chicken that is not found in rodents. Chicken CD8 is expressed on approximately 80% of thymocytes, 15% of blood mononuclear cells, and 50% of spleen cells but less than 1% of cells in the bursa and bone marrow. The monoclonal antibody CT-8 recognizes the CD8alpha chain and has been shown to react to a polymorphic determinant in turkey.