CD8a Antibody [EP72] (FITC) | 99-307

CD8a Antibody [EP72] (FITC) | 99-307 | Gentaur UK, US & Europe Distribution

Host: Mouse

Reactivity: Chicken

Homology: N/A

Immunogen: Chicken splenocytes

Research Area: Cancer, Immunology, Signal Transduction

Tested Application: WB, IHC-Fr, Flow, Blocking, IP

Application: CD8a Antibody [EP72] for use in flow cytometry and immunohistochemistry assays.

Specificiy: CD8alpha

Positive Control 1: N/A

Positive Control 2: N/A

Positive Control 3: N/A

Positive Control 4: N/A

Positive Control 5: N/A

Positive Control 6: N/A

Molecular Weight: N/A

Validation: N/A

Isoform: N/A

Purification: N/A

Clonality: Monoclonal

Clone: [EP72]

Isotype: IgG2b

Conjugate: FITC

Physical State: Liquid

Buffer: Supplied in PBS/NaN3

Concentration: 0.5 mg/mL

Storage Condition: Store vial at 2-8˚C

Alternate Name: N/A

User Note: Optimal dilutions for each application to be determined by the researcher.

BACKGROUND: In the chicken, the CD8 molecule is present in two forms - (i) a homodimer of two alpha chains and (ii) a heterodimer of an alpha chain and a beta chain. Chicken CD8 is expressed on approximately 80% of thymocytes, 15% of blood mononuclear cells and 50% of spleen cells but less than 1% of cells in the bursa and bone marrow. While the vast majority of CD8+ cells in the thymus, spleen, and blood of adult chickens express both CD8alpha- and CD8beta-chains, a relatively large proportion of the CD8+ TCRgammadelta cells in the spleens of embryos and young chicks express only the alpha-chain of CD8. Among intestinal epithelial lymphocytes, the major CD8+ T cell populations present in mice are conserved but there is a population of TCRgammadelta CD8alphabeta cells in the chicken that is not found in rodents. The monoclonal antibody EP72 recognizes the CD8alpha chain.