CD8B Antibody (N-term) [APR31244G]

CD8B Antibody (N-term) [APR31244G] | Gentaur UK, US & Europe Distribution

Product Category: Polyclonal Antibodies

Host: Rabbit

Species Reactivity: H

Specificity: This CD8B antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 64-92 amino acids from the N-terminal region of human CD8B.

Cellular Localisation: [Isoform 1]: Cell membrane; Single-pass type I membrane protein. Note=Requires the partner CD8A for efficient cell surface expression (PubMed:3145196) . The heterodimer CD8A/CD8B localizes to lipid rafts due to CD8B cytoplasmic tail palmitoylation. [Isoform 3]: Secreted. [Isoform 5]: Cell membrane; Single- pass type I membrane protein [Isoform 7]: Secreted.

Molecular Weight: 23722

Clone: Polyclonal

Gene Name: CD8B

Gene ID: 926

Function: Integral membrane glycoprotein that plays an essential role in the immune response and serves multiple functions in responses against both external and internal offenses. In T-cells, functions primarily as a coreceptor for MHC class I molecule:peptide complex. The antigens presented by class I peptides are derived from cytosolic proteins while class II derived from extracellular proteins. Interacts simultaneously with the T-cell receptor (TCR) and the MHC class I proteins presented by antigen presenting cells (APCs) . In turn, recruits the Src kinase LCK to the vicinity of the TCR-CD3 complex. A palmitoylation site in the cytoplasmic tail of CD8B chain contributes to partitioning of CD8 into the plasma membrane lipid rafts where signaling proteins are enriched. Once LCK recruited, it initiates different intracellular signaling pathways by phosphorylating various substrates ultimately leading to lymphokine production, motility, adhesion and activation of cytotoxic T-lymphocytes (CTLs) . Additionally, plays a critical role in thymic selection of CD8+ T- cells.

Summary: Tissue Location: Isoform 1, isoform 3, isoform 5, isoform 6, isoform 7 and isoform 8 are expressed in both thymus and peripheral CD8+ T- cells. Expression of isoform 1 is higher in thymus CD8+ T-cells than in peripheral CD8+ T-cells. Expression of isoform 6 is higher in peripheral CD8+ T-cells than in thymus CD8+ T-cells

Form: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.

Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

Application: WB, FC

Dilution: WB--1:1000 FC--1:10~50

Synonyms: T-cell surface glycoprotein CD8 beta chain, CD8b, CD8B, CD8B1