Description
Cdc42-binding kinase alpha Antibody | 45-384 | Gentaur UK, US & Europe Distribution
Host: Goat
Reactivity: Human
Homology: Expected Species Reactivity based on sequence homology: Mouse, Rat, Dog, Cow
Immunogen: The immunogen for this antibody is: SGEVRLRQLEQFI-C
Research Area: Cancer
Tested Application: E, IHC
Application: Peptide ELISA: antibody detection limit dilution 1:16000.Western Blot:Preliminary experiments gave an approx 30kDa and 40kDa bands in Human Pancreas lysates at 1ug/ml. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated sizes of 186kDa according to NP_055641.3 and 196kDa according to NP_003598.2. We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates? Have any further splice variants/modified forms been reported?Immunohistochemistry:In paraffin embedded Human Cerebellum shows cytoplasm staining in purkinje cells. Recommended concentration, 3-6ug/ml.
Specificiy: This antibody is expected to recognise both reported Human isoforms (NP_055641.3 and NP_003598.2) .
Positive Control 1: N/A
Positive Control 2: N/A
Positive Control 3: N/A
Positive Control 4: N/A
Positive Control 5: N/A
Positive Control 6: N/A
Molecular Weight: N/A
Validation: N/A
Isoform: N/A
Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality: Polyclonal
Clone: N/A
Isotype: N/A
Conjugate: Unconjugated
Physical State: Liquid
Buffer: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration: 500 ug/mL
Storage Condition: Aliquot and store at -20˚C. Minimize freezing and thawing.
Alternate Name: CDC42BPA, MRCK, MRCKA, PK428, FLJ23347, KIAA0451, CDC42 binding protein kinase alpha (DMPK-like) , ser-thr protein kinase PK428, myotonic dystrophy kinase-related CDC42-binding protein kinase alpha, Ser-Thr protein kinase related to the myotonic dystrophy protein kinase, DKFZp686L1738, CDC42 binding protein kinase beta
User Note: N/A
BACKGROUND: N/A