Cdk1/Cdc2 monoclonal Antibody | MB0019

Cdk1/Cdc2 monoclonal Antibody | MB0019 | Gentaur UK, US & Europe Distribution

Host: Mouse

Reactivity: Human

Application: WB ICC

Application Range: WB: 1:1000 ICC: 1:50~200

Background: Cdc2, an evolutionarily conserved serine/threonine-specific protein kinase, is essential in the cell cycle transition from G2 to M phase. Cdc2 is regulated by association with B-type cyclins and by reversible phosophorylation. Cyclin B binding facilitates the phosphorylation of Cdc2 p34 on three regulatory sites: threonine 14, tyrosine 15, and threonine 161. In higher eukaryotes, Cdc2 is negatively regulated by phosphorylation of two residues located in the ATP-binding site, Thr 14 and Tyr 15. Cdc2 is positively regulated by the cyclin-dependent phosphorylation of Thr 161. Both phosphorylation and de- phosphorylation at Thr 161 are required for progression through the cell cycle.

Storage & Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: This Antibody detects endogenous levels of Cdk1/Cdc2 and does not cross-react with related proteins

Molecular Weight: Predicted band size:34KDa Observed band size:34KDa

Note: For research use only, not for use in diagnostic procedure.

Alternative Names: Cyclin-dependent kinase 1; Cell division control protein 2 homolog; Cell division protein kinase 1; p34 protein kinase; CDK1; CDC2; CDC28A; CDKN1; P34CDC2

Immunogen: Recombinant full length Human Cdk1/Cdc2.

Conjugate: Unconjugated

Modification: Unmodification

Purification & Purity: The Antibody was affinity-purified from mouse ascites by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .

Pathway: