Cdk1/Cdc2 (phospho-Y15) polyclonal Antibody | BS5058

Cdk1/Cdc2 (phospho-Y15) polyclonal Antibody | BS5058 | Gentaur UK, US & Europe Distribution

Host: Rabbit

Reactivity: Human,Mouse,Rat

Application: WB IHC

Application Range: WB: 1:500~1:1000 IHC: 1:50~1:200

Background: Cdc2, an evolutionarily conserved serine/threonine-specific protein kinase, is essential in the cell cycle transition from G2 to M phase. Cdc2 is regulated by association with B-type cyclins and by reversible phosophorylation. Cyclin B binding facilitates the phosphorylation of Cdc2 p34 on three regulatory sites: threonine 14, tyrosine 15, and threonine 161. In higher eukaryotes, Cdc2 is negatively regulated by phosphorylation of two residues located in the ATP-binding site, Thr 14 and Tyr 15. Cdc2 is positively regulated by the cyclin-dependent phosphorylation of Thr 161. Both phosphorylation and de- phosphorylation at Thr 161 are required for progression through the cell cycle.

Storage & Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: p-Cdk1/Cdc2 (Y15) polyclonal Antibody detects endogenous levels of Cdk1/Cdc2 protein only when phosphorylated at Tyr15.

Molecular Weight: ~ 34 kDa

Note: For research use only, not for use in diagnostic procedure.

Alternative Names: Cyclin-dependent kinase 1; Cell division control protein 2 homolog; Cell division protein kinase 1; p34 protein kinase; CDK1; CDC2; CDC28A; CDKN1; P34CDC2

Immunogen: Synthetic phosphopeptide derived from human Cdk1/Cdc2 around the phosphorylation site of Serine 795.

Conjugate: Unconjugated

Modification: Phosphorylation

Purification & Purity: The Antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .

Pathway: Cell Cycle Control G1 S Checkpoint,Cell Cycle G2 M DNA Damage Signaling Pathway,Epigenetic Writers and Erasers of Histone H3,