CDK4 monoclonal Antibody | MB0027

CDK4 monoclonal Antibody | MB0027 | Gentaur UK, US & Europe Distribution

Host: Mouse

Reactivity: Human,Mouse

Application: WB

Application Range: WB: 1:1000

Background: Ser/Thr-kinase component of cyclin D-CDK4 (DC) complexes that phosphorylate and inhibit members of the retinoblastoma (RB) protein family including RB1 and regulate the cell-cycle during G1/S transition. Phosphorylation of RB1 allows dissociation of the transcription factor E2F from the RB/E2F complexes and the subsequent transcription of E2F target genes which are responsible for the progression through the G1 phase. Hypophosphorylates RB1 in early G1 phase. Cyclin D-CDK4 complexes are major integrators of various mitogenenic and antimitogenic signals. Also phosphorylates SMAD3 in a cell-cycle-dependent manner and represses its transcriptional activity. Component of the ternary complex, cyclin D/CDK4/CDKN1B, required for nuclear translocation and activity of the cyclin D-CDK4 complex.

Storage & Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: This Antibody detects endogenous levels of CDK4 and does not cross-react with related proteins

Molecular Weight: Predicted band size:34KDa Observed band size:33KDa

Note: For research use only, not for use in diagnostic procedure.

Alternative Names: Cyclin-dependent kinase 4; Cell division protein kinase 4; PSK-J3; PSKJ3; CDK4

Immunogen: Recombinant full length Human CDK4.

Conjugate: Unconjugated

Modification: Unmodification

Purification & Purity: The Antibody was affinity-purified from mouse ascites by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .

Pathway: