CDX2 Antibody | 5595

CDX2 Antibody | 5595 | Gentaur UK, US & Europe Distribution

Host: Rabbit

Reactivity: Human, Mouse, Rat

Homology: Predicted species reactivity based on immunogen sequence: Chicken: (94%) , Bovine: (90%)

Immunogen: CDX2 antibody was raised against a 16 amino acid synthetic peptide near the carboxy terminus of human CDX2.
The immunogen is located within amino acids 220 - 270 of CDX2.

Research Area: Homeostasis, Stem Cell

Tested Application: E, WB, IHC-P, IF

Application: CDX2 antibody can be used for detection of CDX2 by Western blot at 1 μg/mL. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in mouse samples; Immunohistochemistry in rat samples and Immunofluorescence in rat samples. All other applications and species not yet tested.

Specificiy: N/A

Positive Control 1: Cat. No. 1403 - Mouse Brain Tissue Lysate

Positive Control 2: N/A

Positive Control 3: N/A

Positive Control 4: N/A

Positive Control 5: N/A

Positive Control 6: N/A

Molecular Weight: N/A

Validation: N/A

Isoform: N/A

Purification: CDX2 Antibody is affinity chromatography purified via peptide column.

Clonality: Polyclonal

Clone: N/A

Isotype: IgG

Conjugate: Unconjugated

Physical State: Liquid

Buffer: CDX2 Antibody is supplied in PBS containing 0.02% sodium azide.

Concentration: 1 mg/mL

Storage Condition: CDX2 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Alternate Name: CDX2 Antibody: CDX3, CDX-3, CDX3, Homeobox protein CDX-2

User Note: Optimal dilutions for each application to be determined by the researcher.

BACKGROUND: CDX2 Antibody: CDX2 is a member of the caudal homeobox family of transcription factors that also includes the proteins CDX1 and CDX4. CDX2 is strongly expressed in the trophectoderm in the blastocyst with expression maintained within the proliferating extra-embryonic ectoderm during development. It is required for the repression of the transcription of the POU5F1/Oct4 and NANOG genes in the outer cells of the blastocyst and is thus essential for the segregation of the inner cell mass and trophectoderm at the blastocyst stage. Aberrant expression of CDX2 is associated with numerous types of carcinomas, including intestinal metaplasia, gastric carcinoma, and acute myeloid leukemia due to deregulated HOX gene expression.