CheKine™ Advanced Oxidation Protein Products (AOPP) Assay Kit（Colorimetric） | KTB1060

CheKine™ Advanced Oxidation Protein Products (AOPP) Assay Kit（Colorimetric） | KTB1060 | Gentaur UK, US & Europe Distribution

Product Category: Cytology

Application: Cell Metabolism

Product Type: Biochemical Assay Kit

Sequence: N/A

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protein Lenght: N/A

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Kit Component: • Ferrous Salt
• H2O2
•Salicylic Acid

• Free-HSA Negative Control

Features & Benefits : • simple, sensitive, rapid detection method.
• compatible to various biological samples such as plasma, serum, bacteria, animal /plant tissues and cells and other biological fluids.
• Provides detailed sample preparation, results calculation methods and comprehensive results display.

Molecular Weight: N/A

Usage Note: • Do not mix the components between different batch numbers and manufacturers, otherwise, the results may be abnormal.
• Avoid bubbles while mixing or redissolving components.
• Change pipette tips frequently to avoid cross contamination between components.
• Ensure that all components and equipment are at the proper temperature before starting the experiment.
• In order to guarantee the accuracy of experimental results, need to do a pre-experiment with 1-2 samples.
• If the OD value exceeds 1.3, the sample needs to be diluted.
• The sample is hemolyzed or has a dark color and needs to be compared with itself. The specific method is to add 200 µL of the diluted sample and 30 µL of Extraction Buffer (1x) to UV microplate, incubate at 37°C for 5 min, record the absorbance at 340 nm, and subtract the OD value of the self-control from the measurement well during calculation.
• Serum, plasma and tissue samples are recommended to be diluted 5 times with Extraction Buffer (1x) for testing.
• The Reagent I and Reagent II are stored in sealed condition.

Storage Conditions: Storage at 4°C. Kit has a storage time of 12 months from receipt. Please refer to the table below Materials supplied and Storage conditions to store all the components.

Shipping: Gel pack with blue ice.

Background: Abbkine CheKine™ Hydroxyl Free Radical Scavenging Capacity Assay Kit (Colorimetric) (Salicylic Acid Method) is specially developed for the detection of Hydroxyl Free Radical Scavenging Capacity in various sample. The operation is simple and convenient, and the detection is more sensitive and accurate. In this assay, H2O2/ Fe2+ generates hydroxyl free radical through the Fenton reaction. Salicylic acid can effectively capture the generated hydroxyl free radical and reacts with them to produce 2, 3- dihydroxybenzoic acid with a maximum absorption peak at 520 nm. After the substances with the capacity to scavenge hydroxyl free radical, resulting in the decrease of 520 nm absorbance. The value of 520 nm absorbance can reflects the Hydroxyl Free Radical Scavenging Capacity of the sample.

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Tag: Hydroxyl Free Radical Scavenging Capacity