Chicken APEX1 (Apurinic/Apyrimidinic Endonuclease 1) ELISA Kit | ELK8968

Chicken APEX1 (Apurinic/Apyrimidinic Endonuclease 1) ELISA Kit | ELK8968 | Gentaur UK, US & Europe Distribution

Alternative Names: APE; APE1; APEN; APEX; APX; HAP1; REF1; Multifunctional DNA Repair Enzyme; APEX Nuclease 1; Redox factor-1; DNA- (apurinic or apyrimidinic site) lyase

Species: Chicken

Assay Type: Sandwich

Sensitivity: 0.152 ng/mL

Standard: 20 ng/mL

Detection Range: 0.32-20 ng/mL

Sample Type: tissue homogenates, cell lysates and other biological fluids

Assay Lenght: 3.5h

Research Area: Enzyme & Kinase

Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Apurinic/Apyrimidinic Endonuclease 1 (APEX1) . Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Apurinic/Apyrimidinic Endonuclease 1 (APEX1) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Apurinic/Apyrimidinic Endonuclease 1 (APEX1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Apurinic/Apyrimidinic Endonuclease 1 (APEX1) in the samples is then determined by comparing the OD of the samples to the standard curve.