Chicken COL9a1 (Collagen Type IX Alpha 1) ELISA Kit | ELK9438

Chicken COL9a1 (Collagen Type IX Alpha 1) ELISA Kit | ELK9438 | Gentaur UK, US & Europe Distribution

Alternative Names: COL9-A1; MED; Collagen Alpha-1 (IX) chain

Species: Chicken

Assay Type: Sandwich

Sensitivity: 0.58 ng/mL

Standard: 100 ng/mL

Detection Range: 1.57-100 ng/mL

Sample Type: Tissue homogenates and other biological fluids.

Assay Lenght: 3.5h

Research Area: Metabolic pathway;Ophthalmology & Otorhinolaryngology;Developmental science;Nutrition metabolism;Rheumatology;Autoimmunity

Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Collagen Type IX Alpha 1 (COL9a1) . Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Collagen Type IX Alpha 1 (COL9a1) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Collagen Type IX Alpha 1 (COL9a1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Collagen Type IX Alpha 1 (COL9a1) in the samples is then determined by comparing the OD of the samples to the standard curve.