Chicken HPX (Hemopexin) ELISA Kit | ELK7563

Chicken HPX (Hemopexin) ELISA Kit | ELK7563 | Gentaur UK, US & Europe Distribution

Alternative Names: Haemopexin; Beta-1B-glycoprotein

Species: Chicken

Assay Type: Sandwich

Sensitivity: 2.69 ng/mL

Standard: 400 ng/mL

Detection Range: 6.25-400 ng/mL

Sample Type: Serum, plasma and other biological fluids

Assay Lenght: 3.5h

Research Area: Metabolic pathway;Infection immunity;Hematology

Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Hemopexin (HPX) . Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Hemopexin (HPX) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Hemopexin (HPX), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Hemopexin (HPX) in the samples is then determined by comparing the OD of the samples to the standard curve.