Chicken HSPb2 (Heat Shock Protein Beta 2) ELISA Kit | ELK2431

Chicken HSPb2 (Heat Shock Protein Beta 2) ELISA Kit | ELK2431 | Gentaur UK, US & Europe Distribution

Alternative Names: HSP-B2; MKBP; HSP27; Heat Shock 27kDa Protein 2; DMPK-binding protein

Species: Chicken

Assay Type: Sandwich

Sensitivity: 5.7 pg/mL

Standard: 1000 pg/mL

Detection Range: 15.63-1000 pg/mL

Sample Type: Tissue homogenates and other biological fluids.

Assay Lenght: 3.5h

Research Area: Signal transduction;Tumor immunity;Developmental science

Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Heat Shock Protein Beta 2 (HSPb2) . Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Heat Shock Protein Beta 2 (HSPb2) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Heat Shock Protein Beta 2 (HSPb2), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Heat Shock Protein Beta 2 (HSPb2) in the samples is then determined by comparing the OD of the samples to the standard curve.