Chicken LAMP1 (Lysosomal Associated Membrane Protein 1) ELISA Kit | ELK8922

Chicken LAMP1 (Lysosomal Associated Membrane Protein 1) ELISA Kit | ELK8922 | Gentaur UK, US & Europe Distribution

Alternative Names: CD107-A; CD107a; LAMPA; LGP120; Lysosome-associated membrane protein 1; CD107 antigen-like family member A

Species: Chicken

Assay Type: Sandwich

Sensitivity: 0.055 ng/mL

Standard: 10 ng/mL

Detection Range: 0.16-10 ng/mL

Sample Type: tissue homogenates, cell lysates and other biological fluids

Assay Lenght: 3.5h

Research Area: CD & Adhesion molecule;Tumor immunity;Infection immunity

Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Lysosomal Associated Membrane Protein 1 (LAMP1) . Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Lysosomal Associated Membrane Protein 1 (LAMP1) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Lysosomal Associated Membrane Protein 1 (LAMP1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Lysosomal Associated Membrane Protein 1 (LAMP1) in the samples is then determined by comparing the OD of the samples to the standard curve.