Chicken sIgA (Secretory Immunoglobulin A) ELISA Kit | ELK4908

Chicken sIgA (Secretory Immunoglobulin A) ELISA Kit | ELK4908 | Gentaur UK, US & Europe Distribution

Alternative Names: S-IgA

Species: Chicken

Assay Type: Competitive Inhibition

Sensitivity: 248.5 pg/mL

Standard: 50000 pg/mL

Detection Range: 781.25-50000 pg/mL

Sample Type: Serum, plasma and other biological fluids

Assay Lenght: 2h

Research Area: Infection immunity;Immune molecule;Reproductive science;Genetic science;Dermatology;Immunodeficiency

Test Principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Secretory Immunoglobulin A (sIgA) protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Secretory Immunoglobulin A (sIgA) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Secretory Immunoglobulin A (sIgA) in the samples is then determined by comparing the OD of the samples to the standard curve.