CHK1, Active | 7735

CHK1 is a 56 kd serine/threonine protein kinase that was originally identified in fission yeast to play a role in activation of the DNA damage checkpoint in the G2 phase of the cell cycle (1). CHK1 appears to function downstream of several of the known fission yeast checkpoint gene products, including that encoded by rad3+, a gene with sequence similarity to the ATM gene mutated in patients with ataxia telangiectasia (2). In vitro, CHK1 binds to and phosphorylates the dual-specificity protein phosphatases Cdc25A, Cdc25B, and Cdc25C, which control cell cycle transitions by dephosphorylating cyclin-dependent kinases (3). CHK1 phosphorylates Cdc25C on serine-216 which creates a binding site for 14-3-3 protein and inhibits function of the phosphatase. Thus, in response to DNA damage, CHK1 phosphorylates and inhibits Cdc25C, thereby preventing the activation of the Cdc2-cyclin B complex and mitotic entry.

7735 | CHK1 Active DataSheet

Biomolecule/Target: N/A

Synonyms: CHK, Serine/threonine-protein kinase Chk1

Alternates names: bone morphogenetic protein 3, Osteogenin, BMP-3A

Taglines: Regulates cell cycle checkpoints and apoptosis

NCBI Gene ID #: 651

NCBI Gene Symbol: BMP3

Gene Source: N/A

Accession #: M22491

Recombinant: Yes

Source: Baculovirus (Sf9 insect cells)

Purity by SDS-PAGEs: 98%

Assay: SDS-PAGE

Purity: N/A

Assay #2: HPLC

Endotoxin Level: N/A

Activity (Specifications/test method): 369 nmol phosphate incorporated into CHKtide per minute per mg protein at 30°C for 15 minutes using a final concentration of 50 µM ATP (0.83 Ci/assay).

Biological activity: Testing is in progress.

Results: N/A

Binding Capacity: N/A

Unit Definition: N/A

Molecular Weight: 31.6 kDa

Concentration: 0.1 mg/ml

Appearance: Liquid

Physical form description: Recombinant protein in storage buffer (50 mM Tris-HCl, pH 7.5, 150 mM NaCl, 0.25 mM DTT, 0.1 mM EGTA, 0.1 mM EDTA, 0.1 mM PMSF, 30% glycerol.).

Reconstitution Instructions: Reconstitute to a concentration of 0.1-1.0 mg/ml in HO containing BSA (50 mg BSA per 1 mg of protein). This solution can then be diluted into other aqueous buffers.

Amino acid sequence: N/A