Chk2 Antibody | 2391

Chk2 Antibody | 2391 | Gentaur UK, US & Europe Distribution

Host: Rabbit

Reactivity: Human, Mouse, Rat

Homology: N/A

Immunogen: Chk2 antibody was raised against a synthetic peptide corresponding to amino acids near the amino terminus of human Chk2.
The immunogen is located within the first 50 amino acids of Chk2.

Research Area: Apoptosis

Tested Application: E, WB, ICC, IF

Application: Chk2 antibody can be used for detection of Chk2 by Western blot at 1 μg/mL. Antibody can also be used for immunocytochemistry starting at 1 μg/mL. For immunofluorescence start at 5 μg/mL.
Antibody validated: Western Blot in human samples; Immunocytochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.

Specificiy: N/A

Positive Control 1: Cat. No. 1205 - Jurkat Cell Lysate

Positive Control 2: Cat. No. 1204 - K562 Cell Lysate

Positive Control 3: Cat. No. 17-005 - Jurkat Cell Slide

Positive Control 4: N/A

Positive Control 5: N/A

Positive Control 6: N/A

Molecular Weight: Predicted: 60 kDa
Observed: 60 kDa

Validation: N/A

Isoform: N/A

Purification: Chk2 Antibody is affinity chromatography purified via peptide column.

Clonality: Polyclonal

Clone: N/A

Isotype: IgG

Conjugate: Unconjugated

Physical State: Liquid

Buffer: Chk2 Antibody is supplied in PBS containing 0.02% sodium azide.

Concentration: 1 mg/mL

Storage Condition: Chk2 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Alternate Name: Chk2 Antibody: CDS1, CHK2, LFS2, RAD53, hCds1, HuCds1, PP1425, CDS1, CHK2 checkpoint homolog, Hucds1

User Note: Optimal dilutions for each application to be determined by the researcher.

BACKGROUND: Chk2 Antibody: The p53 tumor-suppressor gene integrates numerous signals that control cell life and death. Several novel molecules involved in p53 signaling, including Chk2, p53R2, p53AIP1, Noxa, PIDD, and PID/MTA2, were recently discovered. The checkpoint kinase Chk2 is the mammalian homologue of yeast Cds1/Rad53. In response to DNA damage, the checkpoint kinase ATM phosphorylates and activates Chk2, which in turn directly phosphorylates and activates p53. Chk2 serves as ATM downstream effector to mediate activation of p53. Chk2 also phosphorylates and activates BRCA1, the product of a tumor suppressor gene that is mutated in breast and ovarian cancer.