Chk2 (phospho Thr68) Polyclonal Antibody | ABP0032

Chk2 (phospho Thr68) Polyclonal Antibody | ABP0032 | Gentaur UK, US & Europe Distribution

Immunogen: Synthesized peptide derived from human Chk2 around the phosphorylation site of T68

Product Category: Protein

Application: Protein General Reagents

Product Type: Selected Primary Antibodies

Host: Rabbit

Reactivity: Human, Mouse, Rat

Application: WB, IF, IHC-P, ELISA

Application Note: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB (1:500-1:2000), IF (1:50-1:200), IHC-P (1:100-1:300), ELISA (1:20000) . Not yet tested in other applications.

Clonality: Polyclonal

Isotype: Rabbit IgG

Formulation: Liquid solution

Kit Component: N/A

Concentration: 1 mg/ml

Storage Buffer: PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide.

Storage Instructions: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Shipping Condition: Gel pack with blue ice.

Background: In response to DNA damage and replication blocks, cell cycle progression is halted through the control of critical cell cycle regulators. Checkpoint kinase 2 encoded by CHEK2 is a cell cycle checkpoint regulator and putative tumor suppressor. It contains a forkhead-associated protein interaction domain essential for activation in response to DNA damage and is rapidly phosphorylated in response to replication blocks and DNA damage. When activated, the encoded protein is known to inhibit CDC25C phosphatase, preventing entry into mitosis, and has been shown to stabilize the tumor suppressor protein p53, leading to cell cycle arrest in G1. In addition, Checkpoint kinase 2 interacts with and phosphorylates BRCA1, allowing BRCA1 to restore survival after DNA damage. Mutations in this gene have been linked with Li-Fraumeni syndrome, a highly penetrant familial cancer phenotype usually associated with inherited mutations in TP53. Also, mutations in this gene are thought to confer a predisposition to sarcomas, breast cancer, and brain tumors. This nuclear protein is a member of the CDS1 subfamily of serine/threonine protein kinases. Several transcript variants encoding different isoforms have been found for CHEK2.

Alternative Names: CHEK2; CDS1; CHK2; RAD53; Serine/threonine-protein kinase Chk2; CHK2 checkpoint homolog; Cds1 homolog; Hucds1; hCds1; Checkpoint kinase 2

Precaution: The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.