Chlortetracycline ELISA Kit - E4782

E4782 | Chlortetracycline ELISA Kit DataSheet

Chlortetracycline is an antibiotic produced by some strains of Streptomyces aureofaciens. It inhibits protein synthesis (elongation) by preventing binding of aminoacyl-tRNA to the 30S subunit. It is effective against Gram-positive and to a lesser degree Gram-negative bacteria than tetracycline. Chlortetracycline ELISA Kit is based on Competitive ELISA principle. The micro-plate provided in this kit has been pre-coated with Chlortetracycline. During the reaction, Chlortetracycline in the samples or standard competes with Chlortetracycline coated on the plate for binding to the anti-Chlortetracycline antibody. Then Horseradish Peroxidase (HRP) conjugate is added to each micro plate well, and TMB substrate is for color development. There is a negative correlation between the OD value of samples and the concentration of Chlortetracycline. The concentration of Chlortetracycline in the samples can be calculated by comparing the OD of the samples to the standard curve.

Alternate Name :

Tag Line : A Competitive ELISA kit for in vitro quantitative determination of Chlortetracycline concentrations in Tissue, Liver, Eggs, Honey, Urine.

Summary :

Detection Method : Absorbance (450 nm)

Sample Type : Tissue, Liver, Eggs, Honey, Urine

Species Reactivity : Universal

Applications : in vitro quantitative determination of Chlortetracycline concentrations in Tissue, Liver, Eggs, Honey, Urine

Features and Benefits : Sample recovery rate: Tissue, Liver, Eggs -90%±20%; Honey -75%±20%; Urine -80±20%, Detection range: Tissue, Liver, Eggs - 0.8 ppb, Honey - 4 ppb, Urine -1 ppb, Sensitivity: 0.1 ppb, Cross-reactivity: Chlortetracycline - 100%, Tetracycline -29%, Oxytetracycline -15%, Doxycycline -2.5%