CHMP4A Antibody (C-term) [APR15422G]

CHMP4A Antibody (C-term) [APR15422G] | Gentaur UK, US & Europe Distribution

Product Category: Polyclonal Antibodies

Host: Rabbit

Species Reactivity: H

Specificity: This CHMP4A antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 205-239 amino acids from the C-terminal region of human CHMP4A.

Cellular Localisation: Cytoplasmic vesicle membrane. Late endosome membrane; Peripheral membrane protein Note=Membrane-associated. Localizes to large vesicle-like structures Localizes to the midbody of dividing cells. Localized in two distinct rings on either side of the Fleming body

Molecular Weight: 25098

Clone: Polyclonal

Gene Name: CHMP4A

Gene ID: 29082

Function: Probable core component of the endosomal sorting required for transport complex III (ESCRT-III) which is involved in multivesicular bodies (MVBs) formation and sorting of endosomal cargo proteins into MVBs. MVBs contain intraluminal vesicles (ILVs) that are generated by invagination and scission from the limiting membrane of the endosome and mostly are delivered to lysosomes enabling degradation of membrane proteins, such as stimulated growth factor receptors, lysosomal enzymes and lipids. The MVB pathway appears to require the sequential function of ESCRT-O, -I, -II and -III complexes. ESCRT-III proteins mostly dissociate from the invaginating membrane before the ILV is released. The ESCRT machinery also functions in topologically equivalent membrane fission events, such as the terminal stages of cytokinesis and the budding of enveloped viruses (HIV-1 and other lentiviruses) . ESCRT-III proteins are believed to mediate the necessary vesicle extrusion and/or membrane fission activities, possibly in conjunction with the AAA ATPase VPS4. When overexpressed, membrane-assembled circular arrays of CHMP4A filaments can promote or stabilize negative curvature and outward budding. Via its interaction with PDCD6IP involved in HIV-1 p6- and p9-dependent virus release. CHMP4A/B/C are required for the exosomal release of SDCBP, CD63 and syndecan (PubMed:22660413) .

Summary: Tissue Location: Widely expressed. Expressed at higher level in heart, kidney, liver and skeletal muscle. Also expressed in brain, placenta, lung and pancreas.

Form: N/A

Storage: Store at +4°C short term. For long-term storage, aliquot and store at -20°C or below. Stable for 12 months at -20°C. Avoid repeated freeze-thaw cycles.

Application: WB

Dilution: WB--1:1000

Synonyms: Charged multivesicular body protein 4a, Chromatin-modifying protein 4a, CHMP4a, SNF7 homolog associated with Alix-2, SNF7-1, hSnf-1, Vacuolar protein sorting-associated protein 32-1, Vps32-1, hVps32-1, CHMP4A, C14orf123, SHAX2