Choline Kinase B, Human Recombinant | P1220

Choline Kinase (E.C. 2.7.1.32) is an enzyme which catalyzes the first reaction in the choline pathway for the synthesis of the membrane phospholipid phosphatidylcholine. It belongs to the family of transferase and uses Mg2+ as a cofactor to transfer a phosphate group from ATP to choline to form phosphocholine. Choline Kinase plays a critical role in cell growth. In humans, choline kinase A and B are present and they share >60% homology. Both of them are implicated in different human diseases as choline kinase A is suggested to associate with breast, lung, colorectal, prostate and bladder cancers due to its high expression in different cancer tissues whereas choline kinase B is suggested to be involved in muscular dystrophy. ’s active human choline kinase B is suitable for functional assays, high-throughput screening and preclinical studies in drug discovery.

P1220 | Choline Kinase B Human Recombinant DataSheet

Biomolecule/Target: CHKB

Synonyms: CHKB, choline kinase beta, ATP:choline phosphotransferase B

Alternates names: bone morphogenetic protein 3, Osteogenin, BMP-3A

Taglines: Plays a key role in phospholipid biosynthesis

NCBI Gene ID #: 651

NCBI Gene Symbol: BMP3

Gene Source: Human

Accession #: M22491

Recombinant: Yes

Source: E.coli

Purity by SDS-PAGEs: 98%

Assay: SDS-PAGE

Purity: N/A

Assay #2: N/A

Endotoxin Level: N/A

Activity (Specifications/test method): Measured by the amount of enzyme that catalyzes the phosphorylation of 1 mole of choline to phosphocholine per minute at pH 8 and 37°C.

Biological activity: Testing is in progress.

Results: 0.05 unit/mg

Binding Capacity: N/A

Unit Definition: N/A

Molecular Weight: 31.6 kDa

Concentration: N/A

Appearance: Liquid

Physical form description: In 20 mM Tris, pH 8.0, 150 mM NaCl, 2 mM DTT, 2 mM EDTA and 20% glycerol

Reconstitution Instructions: Reconstitute to a concentration of 0.1-1.0 mg/ml in HO containing BSA (50 mg BSA per 1 mg of protein). This solution can then be diluted into other aqueous buffers.

Amino acid sequence: N/A