CHRNA4 Antibody | 42-755

CHRNA4 Antibody | 42-755 | Gentaur UK, US & Europe Distribution

Host: Goat

Reactivity: Human, Rat

Homology: Expected Species Reactivity based on sequence homology: Mouse, Dog, Cow

Immunogen: The immunogen for this antibody is: C-HVETRAHAEERLLKK

Research Area: Other

Tested Application: E, WB

Application: Peptide ELISA: antibody detection limit dilution 1:64000.Western Blot:Approx 70kDa band observed in Rat Brain lysates (calculated MW of 70.0kDa according to Human NP_000735.1and of 70.2kDa according to Rat NP_077330.1) . Recommended concentration: 1-3ug/ml. An additional band of unknown identity was also consistently observed at 22kDa. This band was successfully blocked by incubation with the immunising peptide. We would appreciate any feedback from people in the field - have any such results been reported with other antibodies/lysates? Have any further splice variants/modified forms been reported? ~75kDa confirmed in both human and rat tissues as the glycoprotein by an anonymous customer.This antibody was shown unfit for immune precipitation.

Specificiy: This antibody is expected NOT to cross-react with the similar alpha 2 subunit.

Positive Control 1: Cat. No. 1463 - Rat Brain Tissue Lysate

Positive Control 2: N/A

Positive Control 3: N/A

Positive Control 4: N/A

Positive Control 5: N/A

Positive Control 6: N/A

Molecular Weight: N/A

Validation: N/A

Isoform: N/A

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Clonality: Polyclonal

Clone: N/A

Isotype: N/A

Conjugate: Unconjugated

Physical State: Liquid

Buffer: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20��C. Minimize freezing and thawing.

Concentration: 500 ug/mL

Storage Condition: Aliquot and store at -20˚C. Minimize freezing and thawing.

Alternate Name: CHRNA4, cholinergic receptor, nicotinic, alpha 4, BFNC, EBN, EBN1, NACRA4, cholinergic receptor, nicotinic, alpha 4 subunit, cholinergic receptor, nicotinic, alpha polypeptide 4, neuronal acetylcholine receptor alpha 4 subunit, neuronal nicotinic acetylcholine receptor alpha-4 subunit

User Note: N/A

BACKGROUND: N/A