CHST1 Antibody (C-term) [APR33064G]

CHST1 Antibody (C-term) [APR33064G] | Gentaur UK, US & Europe Distribution

Product Category: Polyclonal Antibodies

Host: Rabbit

Species Reactivity: H, M, Rat

Specificity: This CHST1 antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 392-426 amino acids from the C-terminal region of human CHST1.

Cellular Localisation: Golgi apparatus membrane; Single- pass type II membrane protein

Molecular Weight: 46715

Clone: Polyclonal

Gene Name: CHST1 (HGNC:1969)

Gene ID: 8534

Function: Sulfotransferase that utilizes 3'-phospho-5'-adenylyl sulfate (PAPS) as sulfonate donor to catalyze the transfer of sulfate to position 6 of internal galactose (Gal) residues of keratan. Cooperates with B4GALT4 and B3GNT7 glycosyltransferases and CHST6 sulfotransferase to construct and elongate disulfated disaccharide unit [->3 (6- sulfoGalbeta) 1->4 (6-sulfoGlcNAcbeta) 1->] within keratan sulfate polymer (PubMed:17690104, PubMed:9405439, PubMed:10642612) . Has a preference for sulfating keratan sulfate, but it also transfers sulfate to the unsulfated polymer (PubMed:9405439) . Involved in biosynthesis of phosphacan, a major keratan sulfate proteoglycan in the developing brain (By similarity) . Involved in biosynthesis of 6-sulfoGalbeta- containing O-linked glycans in high endothelial venules of lymph nodes. May act in a synergistic manner with CHST4 to generate sialyl 6', 6- disulfo Lewis X motif, a recognition determinant for immune cell receptors implicated in leukocyte trafficking (PubMed:10330415) . Catalyzes sulfation of N-acetyllactosamine (LacNAc) oligosaccharides with highest efficiency for sialylated LacNAc structures (PubMed:10642612) .

Summary: Tissue Location: Widely expressed at low level. Expressed in brain and skeletal muscle. Expressed by high endothelial cells (HEVs) and leukocytes.

Form: N/A

Storage: Store at +4°C short term. For long-term storage, aliquot and store at -20°C or below. Stable for 12 months at -20°C. Avoid repeated freeze-thaw cycles.

Application: WB, IHC-P

Dilution: IHC-P--1:100~500 WB--1:2000

Synonyms: N/A