cIAP Antibody | 3325

cIAP Antibody | 3325 | Gentaur UK, US & Europe Distribution

Host: Rabbit

Reactivity: Human, Mouse, Rat

Homology: Predicted species reactivity based on immunogen sequence: Pig: (93%) , Chicken: (92%)

Immunogen: Anti-cIAP antibody (3325) was raised against a peptide corresponding to 14 amino acids near the carboxy terminus of human cIAP. c-IAP antibody detects both c-IAP1 and c-IAP2.
The immunogen is located within amino acids 540-590 of cIAP.

Research Area: Apoptosis, Cancer

Tested Application: E, WB, IHC-P, IF

Application: WB: 1-4 μg/mL; IHC: 10 μg/mL; IF: 20 μg/mL.
Antibody validated: Western Blot in human, mouse and rat samples; Immunohistochemistry in human samples; Immunofluorescence in human samples. All other applications and species not yet tested.

Specificiy: N/A

Positive Control 1: Cat. No. 1331 - Human Lung Tissue Lysate

Positive Control 2: Cat. No. 1203 - A549 Cell Lysate

Positive Control 3: Cat. No. 1220 - SK-N-SH Cell Lysate

Positive Control 4: N/A

Positive Control 5: N/A

Positive Control 6: N/A

Molecular Weight: Predicted: 70kD
Observed: 70kD

Validation:

Independent Antibody Validation in Cell lines (Figure 2) shows similar cIAP expression profile in human, mouse and rat cell lines detected by two independent anti-cIAP antibodies that recognize different epitopes, 3325 against C-terminus domain and competitor antibody against recombinant protein.  cIAP proteins are detected in the most tested cell lines at different expression levels by the two independent antibodies. 

Isoform: Human cIAP has 2 isoforms, including isoform 1 (618aa, 70kD) and isoform 2 (569aa, 64kD) . Mouse cIAP has one isoform (612aa, 70kD) and Rat cIAP also has one isoform (589aa, 67kD) . 3325 can detect human, mouse and rat.

Purification: cIAP Antibody is affinity chromatography purified via peptide column.

Clonality: Polyclonal

Clone: N/A

Isotype: IgG

Conjugate: Unconjugated

Physical State: Liquid

Buffer: cIAP Antibody is supplied in PBS containing 0.02% sodium azide.

Concentration: 1 mg/mL

Storage Condition: cIAP antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Alternate Name: cIAP Antibody: API1, MIHB, HIAP2, RNF48, cIAP1, Hiap-2, c-IAP1, API1, IAP2, Baculoviral IAP repeat-containing protein 2, C-IAP1, IAP-2

User Note: Optimal dilutions for each application to be determined by the researcher.

BACKGROUND: cIAP Antibody: Apoptosis, or programmed cell death, is related to many diseases, such as cancer. Apoptosis is triggered by a variety of stimuli including members in the TNF family and can be prevented by the inhibitor of apoptosis (IAP) proteins. IAP proteins form a conserved gene family that binds to and inhibits cell death proteases. The two isoforms of c-IAP (c-IAP1 and c-IAP2) are structurally related to XIAP, containing 3 baculoviral IAP repeat (BIR) motifs that are essential and sufficient for the binding and inhibition of caspases-3, -7. The c-IAPs can associate with the death receptor TNF-R2, and mediate the ubiquitinization of TRAF2 following the binding of TNF-α by its receptor. Omi, a negative regulator of c-IAP, inhibits its activity by catalytically cleaving c-IAP. Another negative regulator, Smac/DIABLO, acts by enhancing the auto-ubiquitization activity of c-IAP.