CITED2 Antibody | 7067

CITED2 Antibody | 7067 | Gentaur UK, US & Europe Distribution

Host: Rabbit

Reactivity: Human, Mouse, Rat

Homology: Predicted species reactivity based on immunogen sequence: Bovine: (100%)

Immunogen: CITED2 antibody was raised against an 18 amino acid synthetic peptide near the amino terminus of human CITED2.
The immunogen is located within the first 50 amino acids of CITED2.

Research Area: Stem Cell

Tested Application: E, WB, IF

Application: CITED2 antibody can be used for detection of CITED2 by Western blot at 1 - 2 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.

Specificiy: Multiple isoforms of CITED2 are known to exist.

Positive Control 1: Cat. No. 1205 - Jurkat Cell Lysate

Positive Control 2: Cat. No. 17-005 - Jurkat Cell Slide

Positive Control 3: N/A

Positive Control 4: N/A

Positive Control 5: N/A

Positive Control 6: N/A

Molecular Weight: 30 kDa

Validation: N/A

Isoform: N/A

Purification: CITED2 Antibody is affinity chromatography purified via peptide column.

Clonality: Polyclonal

Clone: N/A

Isotype: IgG

Conjugate: Unconjugated

Physical State: Liquid

Buffer: CITED2 Antibody is supplied in PBS containing 0.02% sodium azide.

Concentration: 1 mg/mL

Storage Condition: CITED2 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Alternate Name: CITED2 Antibody: ASD8, MRG1, VSD2, MRG-1, P35SRJ, MSG-related protein 1

User Note: Optimal dilutions for each application to be determined by the researcher.

BACKGROUND: CITED2 Antibody: CITED2 was initially identified as a potential transcriptional activator with significant homology to MSG1, a melanocyte-specific nuclear protein. CITED2 interacts with the histone acetyltransferase p300/CBP and acts as a coactivator to several DNA-binding transcription factors such as HIF-1 and AP-2. CITED2 also controls proliferation of mouse embryonic fibroblasts via the polycomb group genes Bmi-1 and Mel18 and the tumor suppressor Ink4a/Arf. CITED2 has also been found to be an essential regulator of adult hematopoietic stem cells (HSCs) , with conditional deletion of CITED2 in the adult mouse resulting in the loss of HSCs, multilineage bone marrow failure and increased lethality.