CLASP2 Antibody (C-term) [APR32382G]

CLASP2 Antibody (C-term) [APR32382G] | Gentaur UK, US & Europe Distribution

Product Category: Polyclonal Antibodies

Host: Rabbit

Species Reactivity: H

Specificity: This CLASP2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 946-975 amino acids from the C-terminal region of human CLASP2.

Cellular Localisation: Cytoplasm, cytoskeleton. Cytoplasm, cytoskeleton, microtubule organizing center, centrosome. Chromosome, centromere, kinetochore. Cytoplasm, cytoskeleton, spindle. Golgi apparatus {ECO:0000250|UniProtKB:Q8BRT1}. Golgi apparatus, trans-Golgi network. Cell membrane. Cell projection, ruffle membrane. Note=Localizes to microtubule plus ends (PubMed:15631994) . Localizes to centrosomes, kinetochores and the mitotic spindle from prometaphase. Subsequently localizes to the spindle midzone from anaphase and to the midbody from telophase (PubMed:16866869, PubMed:16914514) . In migrating cells localizes to the plus ends of microtubules within the cell body and to the entire microtubule lattice within the lamella. Localizes to the cell cortex and this requires ERC1 and PHLDB2 (PubMed:16824950) . The MEMO1-RHOA- DIAPH1 signaling pathway controls localization of the phosphorylated form to the cell membrane.

Molecular Weight: 141064

Clone: Polyclonal

Gene Name: CLASP2

Gene ID: 23122

Function: Microtubule plus-end tracking protein that promotes the stabilization of dynamic microtubules (PubMed:26003921) . Involved in the nucleation of noncentrosomal microtubules originating from the trans-Golgi network (TGN) . Required for the polarization of the cytoplasmic microtubule arrays in migrating cells towards the leading edge of the cell. May act at the cell cortex to enhance the frequency of rescue of depolymerizing microtubules by attaching their plus-ends to cortical platforms composed of ERC1 and PHLDB2 (PubMed:16824950) . This cortical microtubule stabilizing activity is regulated at least in part by phosphatidylinositol 3-kinase signaling. Also performs a similar stabilizing function at the kinetochore which is essential for the bipolar alignment of chromosomes on the mitotic spindle (PubMed:16866869, PubMed:16914514) . Acts as a mediator of ERBB2- dependent stabilization of microtubules at the cell cortex.

Summary: Tissue Location: Brain-specific.

Form: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.

Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

Application: WB, IHC-P

Dilution: WB--1:1000 IHC-P--1:100~500

Synonyms: CLIP-associating protein 2, Cytoplasmic linker-associated protein 2, Protein Orbit homolog 2, hOrbit2, CLASP2, KIAA0627