Clathrin heavy-chain 1, 2 | AS10 690

Clathrin heavy-chain 1, 2 | AS10 690 | Gentaur UK, US & Europe Distribution

Immunogen: KLH-conjugated peptide derived from available plant clathrin heavy chain sequences including Arabidopsis thaliana clathrin heavy chain 1 UniProt: Q0WNJ6, TAIR:At3g11130, clathrin heavy chain 2 UniProt: Q0WLB5, TAIR:At3g08530

Host: Rabbit

Conjugation: N/A

Clonality: Polyclonal

Isotype: N/A

Purity: Immunogen affinity purified serum in PBS pH 7.4.

Format: Lyophilized

Tested Application: Immunolocalization (IL), Immunofluorescence (IF), Western blot (WB)

Related Products: AS10 680 | Anti-Tubulin alpha chain, rabbit antibodiesAS10 681 | Anti-Tubulin beta chain, rabbit antibodiesPlant and algal protein extraction bufferSecondary antibodies

Recommended Dilutions: 1 : 2400 (IL), 1 :400 (IF), 1 : 2000 (WB)

Molecular weight: 193 | 170 kDa (Arabidopsis thaliana)

Confirmed Reactivity: Arabidopsis thaliana, Chlamydomonas reinhardtii, Nicotiana tabacum

Predicted Reactivity: Amborella trichopoda, Brassica napus, Capsella rubella, Citrus aurantium var. sinensis, Eucalyptus grandis, Glycine max, Chlorella variabilis, Leucaena glauca, Lotus japonicus, Medicago tribuloides, Mimulus guttatus, Musa malaccensis, Oryza sativa, Panicum italicum, Physcomitrium patens, Phaseolus vulgaris, Pisum sativum, Populus balsamifera, Populus trichocarpa, Ricinus communis, Selaginella moellendorffii, Sisymbrium salsugineum, Solanum lycopersicum, Theobroma cacao, Triticum aestivum, Vitis vinifera, Zea mays.Species of your interest not listed? Contact us

Not reactive in: Nicotiana benthamiana

Additional Information: Please, note that fresh samples will provide better restuls (see image below)

Background: Clathrin is a protein involved in intracellular trafficking and plays a major role in the formation of coated vesicles. It consists of three clathrin heavy chains and three light chains. Clathrin-coated vesicles (CCV) selectively sort cargo at the cell membrane, trans-Golgi network, and endosomal compartments for multiple membrane traffic pathways.

Reconstitution: For reconstitution add 50 µl of sterile water

Storage: Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.

TAIR Nnumbre: AT3G11130

Category: Zea mays

Research Area: Cell Biology