Cleaved-CASP3 (Asp175) Antibody [APR07091G]

Cleaved-CASP3 (Asp175) Antibody [APR07091G] | Gentaur UK, US & Europe Distribution

Product Category: Polyclonal Antibodies

Host: Rabbit

Species Reactivity: H

Specificity: This Cleaved antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 149-175 amino acids from human Cleaved.

Cellular Localisation: Cytoplasm.

Molecular Weight: 31608

Clone: Polyclonal

Gene Name: CASP3

Gene ID: 836

Function: Involved in the activation cascade of caspases responsible for apoptosis execution (PubMed:7596430) . At the onset of apoptosis it proteolytically cleaves poly (ADP-ribose) polymerase (PARP) at a '216- Asp-|-Gly-217' bond (PubMed:7774019) . Cleaves and activates sterol regulatory element binding proteins (SREBPs) between the basic helix- loop-helix leucine zipper domain and the membrane attachment domain. Cleaves and activates caspase-6, -7 and -9 (PubMed:7596430) . Involved in the cleavage of huntingtin (PubMed:8696339) . Triggers cell adhesion in sympathetic neurons through RET cleavage (PubMed:21357690) . Cleaves and inhibits serine/threonine-protein kinase AKT1 in response to oxidative stress (PubMed:23152800) . Cleaves XRCC4 and phospholipid scramblase proteins XKR4, XKR8 and XKR9, leading to promote phosphatidylserine exposure on apoptotic cell surface (PubMed:23845944, PubMed:33725486) .

Summary: Tissue Location: Highly expressed in lung, spleen, heart, liver and kidney. Moderate levels in brain and skeletal muscle, and low in testis. Also found in many cell lines, highest expression in cells of the immune system

Form: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.

Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

Application: DB, IHC-P

Dilution: DB--1:500 IHC-P--1:100~500

Synonyms: Caspase-3, CASP-3, Apopain, Cysteine protease CPP32, CPP-32, Protein Yama, SREBP cleavage activity 1, SCA-1, Caspase-3 subunit p17, Caspase-3 subunit p12, CASP3, CPP32