Cleaved-DFF45 (D224) polyclonal Antibody | BS7010

Cleaved-DFF45 (D224) polyclonal Antibody | BS7010 | Gentaur UK, US & Europe Distribution

Host: Rabbit

Reactivity: Human,Mouse,Rat

Application: WB

Application Range: WB: 1:500~1:1000

Background: The CED/ICE family of cysteine proteases plays a pivotal role in mediating apoptosis through the proteolysis of specific targets. Among the targets are poly (ADphospho-ribose) polymerase (PARP), gelsolin, DFF-45/ICAD and the nuclear lamins. PARP is a nuclear protein that is specifically cleaved by CPP32 and Mch2, but not by ICE, into a signature apoptotic fragment. Gelsolin is cleaved by CPP32 to an active form that severs actin filaments in a Ca2+-independent manner. In addition to binding actin, gelsolin can form complexes with fibronectin, which may be important for localizing gelsolin to inflammatory sites. DFF-45/ICAD, the subunit of DNA fragmentation factor, is cleaved by CPP32 to generate an active factor that induces DNA fragmentation. The nuclear Lamin A is cleaved by Mch2, but not CPP32. Nuclear Lamin B is fragmented as a consequence of apoptosis by an unidentified member of the ICE family

Storage & Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: Cleaved-DFF45 (D224) polyclonal Antibody detects endogenous levels of Cleaved-DFF45 (D224) protein.

Molecular Weight: ~ 35, 45 kDa

Note: For research use only, not for use in diagnostic procedure.

Alternative Names: Endonuclease 8-like 3; DNA glycosylase FPG2; DNA glycosylase/AP lyase Neil3; Endonuclease VIII-like 3; Nei-like protein 3; NEIL3

Immunogen: Synthetic peptide, corresponding Human NEIL3.

Conjugate: Unconjugated

Modification: Cleaved

Purification & Purity: The Antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .

Pathway: